Organogenesis from ‘Passe Crassane’ and ‘Old Home’ pear ( Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants
Identifieur interne : 000257 ( France/Analysis ); précédent : 000256; suivant : 000258Organogenesis from ‘Passe Crassane’ and ‘Old Home’ pear ( Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants
Auteurs : J. Ochatt [France] ; Elisabeth Chevreau [France] ; Michel Gallet [France]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1992-05-01.
Abstract
Summary: Large numbers of highly viable mesophyll protoplasts were isolated from shoot cultures of the scion cv ‘Passe Crassane’ and the rootstock genotype ‘Old Home’ of common pear (Pyrus communis L.). Protoplasts were cultured for both genotypes either as liquid layers or as liquid-over-agar cultures, in ammonium-free MS medium with 0.5 M mannitol, 50 mg/l casein enzymatic hydrolysate (CEH), 2.0 mg/l NAA and 1.0 mg/l BAP, plus either 0.5 mg/l IAA (for ‘Old Home’) or 2.0 mg/l IAA (for ‘Passe Crassane’). Protoplast microcalli, obtained by day 60 (‘Passe Crassane’) or day 80 (‘Old Home’), were transferred for further growth to ammonium-free MS medium with 2.0 mg/l NAA and 1.0 mg/l BAP. Shoot bud regeneration from the protoplastderived callus was first attempted between 100 (‘Passe Crassane’) and 120 (‘Old Home’) days after protoplast isolation. For ‘Passe Crassane’, shoot buds were regenerated (day 130) on a half-strength MS medium with 0.1 mg/l IBA, 0.5 mg/l BAP, 50 mg/l CEH and 20 mg/l Ca-panthotenate. For ‘Old Home’, shoot but regeneration only occurred 30 days later and on the same medium as above, which was additionally supplemented with double the concentration of the group B vitamins found in the original MS formulation and 0.05 mg/l GA3. Following micropropagation and in vitro rooting of shoots, the plants were transferred to soil following standard procedures. Trueness-to-type of the regenerated plants was assessed by analysing their leaf isozyme banding profiles (for EST, AP, PRX, SOD, ENP, LAP, PGI, AAT, ADH, MDH and PGM) and comparing them to those corresponding to the original shoots that provided the protoplasts. No differences between the mother shoots and the protoclones were observed for any one of the 11 isozyme systems studied.
Url:
DOI: 10.1007/BF00232965
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000880
- to stream Istex, to step Curation: 000880
- to stream Istex, to step Checkpoint: 001137
- to stream Main, to step Merge: 003C88
- to stream Main, to step Curation: 003980
- to stream Main, to step Exploration: 003980
- to stream France, to step Extraction: 000257
Links to Exploration step
ISTEX:F32DEF9D29804CF3894099AEE0ADAB8541F06CF5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Organogenesis from ‘Passe Crassane’ and ‘Old Home’ pear ( Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants</title><author><name sortKey="Ochatt, J" sort="Ochatt, J" uniqKey="Ochatt J" first="J." last="Ochatt">J. Ochatt</name></author><author><name sortKey="Chevreau, Elisabeth" sort="Chevreau, Elisabeth" uniqKey="Chevreau E" first="Elisabeth" last="Chevreau">Elisabeth Chevreau</name></author><author><name sortKey="Gallet, Michel" sort="Gallet, Michel" uniqKey="Gallet M" first="Michel" last="Gallet">Michel Gallet</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F32DEF9D29804CF3894099AEE0ADAB8541F06CF5</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1007/BF00232965</idno><idno type="url">https://api.istex.fr/document/F32DEF9D29804CF3894099AEE0ADAB8541F06CF5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000880</idno><idno type="wicri:Area/Istex/Curation">000880</idno><idno type="wicri:Area/Istex/Checkpoint">001137</idno><idno type="wicri:doubleKey">0040-5752:1992:Ochatt J:organogenesis:from:passe</idno><idno type="wicri:Area/Main/Merge">003C88</idno><idno type="wicri:Area/Main/Curation">003980</idno><idno type="wicri:Area/Main/Exploration">003980</idno><idno type="wicri:Area/France/Extraction">000257</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Organogenesis from ‘Passe Crassane’ and ‘Old Home’ pear ( Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants</title><author><name sortKey="Ochatt, J" sort="Ochatt, J" uniqKey="Ochatt J" first="J." last="Ochatt">J. Ochatt</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>I.N.R.A., Station d'Amélioration des Espèces Fruitières et Ornementales, Route Georges Morel, 49070, Beaucouzé, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Chevreau, Elisabeth" sort="Chevreau, Elisabeth" uniqKey="Chevreau E" first="Elisabeth" last="Chevreau">Elisabeth Chevreau</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>I.N.R.A., Station d'Amélioration des Espèces Fruitières et Ornementales, Route Georges Morel, 49070, Beaucouzé, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author><author><name sortKey="Gallet, Michel" sort="Gallet, Michel" uniqKey="Gallet M" first="Michel" last="Gallet">Michel Gallet</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>I.N.R.A., Station d'Amélioration des Espèces Fruitières et Ornementales, Route Georges Morel, 49070, Beaucouzé, Angers</wicri:regionArea><placeName><settlement type="city">Angers</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Theoretical and Applied Genetics</title><title level="j" type="sub">International Journal of Plant Breeding Research</title><title level="j" type="abbrev">Theoret. Appl. Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1992-05-01">1992-05-01</date><biblScope unit="volume">83</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1013">1013</biblScope><biblScope unit="page" to="1018">1018</biblScope></imprint><idno type="ISSN">0040-5752</idno></series><idno type="istex">F32DEF9D29804CF3894099AEE0ADAB8541F06CF5</idno><idno type="DOI">10.1007/BF00232965</idno><idno type="ArticleID">BF00232965</idno><idno type="ArticleID">Art13</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Large numbers of highly viable mesophyll protoplasts were isolated from shoot cultures of the scion cv ‘Passe Crassane’ and the rootstock genotype ‘Old Home’ of common pear (Pyrus communis L.). Protoplasts were cultured for both genotypes either as liquid layers or as liquid-over-agar cultures, in ammonium-free MS medium with 0.5 M mannitol, 50 mg/l casein enzymatic hydrolysate (CEH), 2.0 mg/l NAA and 1.0 mg/l BAP, plus either 0.5 mg/l IAA (for ‘Old Home’) or 2.0 mg/l IAA (for ‘Passe Crassane’). Protoplast microcalli, obtained by day 60 (‘Passe Crassane’) or day 80 (‘Old Home’), were transferred for further growth to ammonium-free MS medium with 2.0 mg/l NAA and 1.0 mg/l BAP. Shoot bud regeneration from the protoplastderived callus was first attempted between 100 (‘Passe Crassane’) and 120 (‘Old Home’) days after protoplast isolation. For ‘Passe Crassane’, shoot buds were regenerated (day 130) on a half-strength MS medium with 0.1 mg/l IBA, 0.5 mg/l BAP, 50 mg/l CEH and 20 mg/l Ca-panthotenate. For ‘Old Home’, shoot but regeneration only occurred 30 days later and on the same medium as above, which was additionally supplemented with double the concentration of the group B vitamins found in the original MS formulation and 0.05 mg/l GA3. Following micropropagation and in vitro rooting of shoots, the plants were transferred to soil following standard procedures. Trueness-to-type of the regenerated plants was assessed by analysing their leaf isozyme banding profiles (for EST, AP, PRX, SOD, ENP, LAP, PGI, AAT, ADH, MDH and PGM) and comparing them to those corresponding to the original shoots that provided the protoplasts. No differences between the mother shoots and the protoclones were observed for any one of the 11 isozyme systems studied.</div></front></TEI><affiliations><list><country><li>France</li></country><settlement><li>Angers</li></settlement></list><tree><country name="France"><noRegion><name sortKey="Ochatt, J" sort="Ochatt, J" uniqKey="Ochatt J" first="J." last="Ochatt">J. Ochatt</name></noRegion><name sortKey="Chevreau, Elisabeth" sort="Chevreau, Elisabeth" uniqKey="Chevreau E" first="Elisabeth" last="Chevreau">Elisabeth Chevreau</name><name sortKey="Gallet, Michel" sort="Gallet, Michel" uniqKey="Gallet M" first="Michel" last="Gallet">Michel Gallet</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000257 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000257 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:F32DEF9D29804CF3894099AEE0ADAB8541F06CF5
|texte= Organogenesis from ‘Passe Crassane’ and ‘Old Home’ pear ( Pyrus communis L.) protoplasts and isoenzymatic trueness-to-type of the regenerated plants
}}
| This area was generated with Dilib version V0.6.25. |  |